Fig. 5. Activation and nuclear translocation of Dd-STATc in response to membrane-permeant cyclic nucleotide analogues. (A) The tyrosine phosphorylation of Dd-STATc was determined in untreated cells (cont), cells exposed to 200 mM sorbitol (sorbitol) and in cells exposed to the indicated concentrations of membrane-permeant cyclic nucleotide analogue. Induction was for 3 minutes and the samples were analysed as described in Fig. 2. (B) The activation and (C) the nuclear translocation of Dd-STATc in response to 20 mM 8-bromo-cAMP and 8-bromo-cGMP were determined as described in Fig. 2. The results in panel C are shown as the mean±s.d.