Fig. 1. TSP1/hep I stimulate directed endothelial cell migration. (A) BAE cells were grown to near confluence, loaded with calcein AM, and plated onto a 96-well Neuro Probe chemotaxis chamber coated with vitronectin and fibronectin. Cells were stimulated to migrate towards increasing concentrations of soluble TSP1 for 5 hours. As a positive control, cells were also stimulated to migrate towards a bFGF gradient. Cells were scraped from the upper surface and cells migrating to the lower surface assayed by determining the remaining fluorescence in a plate reader. Migration was normalized to that seen with media alone and presented as fold stimulation of migration above baseline levels. n=4-6, *P<0.05, **P<0.01. (B) Cells were stimulated to migrate towards increasing concentrations of either the hep I peptide or a modified, inactive hep I peptide, and migration was assessed as described in (A). n=3, **P<0.01.