Fig. 2. Hep I increases random migration in endothelial cells. (A) BAE cells were grown to near confluence, loaded with calcein AM, and plated onto a 96-well Neuro Probe chemotaxis chamber coated with vitronectin and fibronectin. Cells were stimulated to migrate in response to increasing concentrations of hep I, or the inactive, modified hep I peptide, in the absence of a gradient for 5 hours. Cells were scraped from the upper surface and cells migrating to the lower surface assayed by determining the remaining fluorescence in a plate reader. Migration was normalized to that seen with media alone and presented as fold stimulation of migration above baseline levels. n=3-4, *P<0.05, **P<0.01. (B) Cells were stimulated to migrate with increasing concentrations of TSP1 in the absence of a gradient and migration was assessed as described in (A). Treatment with 100 pM bFGF was included as a positive control in this assay. n=3, *P<0.05, **P<0.01.