Fig. 3. TSP1/hep I-induced endothelial cell chemotaxis in the Dunn chamber. BAE cells were plated at low density under serum-free conditions onto glass coverslips coated with vitronectin and fibronectin. Cells were allowed to attach for 3 hours and loaded onto the Dunn Chamber in serum-free media. Serum-free media was removed from the outer well and media containing either serum-free media (A), hep I (100 nM) (B), TSP1 (7.8 nM) (C) or modified hep I (100 nM) (D) was added to the outer well. The chamber was sealed, and time-lapse video was taken of the cells over a 7-hour time span. Migration of individual cells was tracked using Metamorph software and individual tracks were analyzed for distance, displacement, directionality and orientation. Final positions of the cells are plotted with the initial point being the origin, and the top of the graph representing the outer well. Results are representative of at least three separate experiments. Average displacement () toward the outer well is given for each treatment. *P<0.05, **P<0.01.