Fig. 4. Histogram for endothelial migration speeds following TSP1/hep I treatment. BAE cells were plated at low density under serum-free conditions onto glass coverslips coated with vitronectin and fibronectin. Cells were allowed to attach for 3 hours and loaded onto the Dunn Chamber in serum-free media (A), or media containing either hep I (100 nM) (B), TSP1 (7.8 nM) (C) or modified hep I (100 nM) (D). The chamber was sealed, and time-lapse video was taken of the cells over a 7-hour time span. Migration of individual cells was tracked using Metamorph software and migration speed was determined. Cells were separated into consecutive speed intervals and the percent of cells migrating at each interval was determined. At least 75 cells were analyzed per condition. n=3-4.