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Fig. 3. Co-elution of BTG2 protein and CCR4 complexes. Cellular extracts from HeLa cells transfected at low efficacy with CCR4His-FLAG- and BTG2FLAG-expressing plasmids were fractionated by gel filtration chromatography using a Superose 6 column, and subjected to immunoblot analysis as described in Fig. 1 using anti-Flag, anti-hCAF1 and anti-hPOP2 antibodies.