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Fig. 1. Chondrogenesis in pellet cultures. Cartilage beads formed by BMSCs cultured under chondrogenic conditions for 1 (a,b), 2 (c,d) and 3 (e,f) weeks. (a,c,e) Toluidine-blue staining. (b,d,f) Alcian-blue staining. Overall morphology, alcianophilia and metachromatic staining with toluidine blue are typical of cartilage. Bar, 100 µm.