Fig. 2. The teneurin-2 intracellular domain colocalizes with PML. Microscopic analysis of HT1080 cells transfected with teneurin-2 variants IT (a), I (b-k), PML (g-i) and PML-RAR (j-l). Staining of non-permeabilized cells with an anti-HA antibody detecting the C-terminal tag of construct IT. (a) Staining of permeabilised cells with anti-VSV detecting the C-terminal tag of construct I (b) and same field analysed in phase contrast (c). Double-immunofluorescence of I transfected cells for I (anti-VSV; d) and endogenous PML (anti-PML; e) with an overlap shown in f. Double immunofluorescence staining of cells co-transfected with I and PML for I (anti-VSV; g) and PML (anti-PML; h) with the overlap shown in i. Double-immunofluorescence staining of cells co-transfected with I and PML-RAR for I (anti-VSV; j) and PML (anti-PML; k). For comparison, l shows PML-RAR single transfected cells (anti-PML). Bar, 50 µm (a-c) or 10 µm (d-l).