Fig. 7. Proteasome inhibitors stabilize the cleaved intracellular domain of teneurin-2. (A) Comparison of luciferase activities induced by transfection of BDAD-teneurin-2 (BDAD-ITEY) in the absence (-) or presence of protease inhibitors (A, ALLN; L, lactacystin). (B) Western blot analysis of ITE- and I-transfected COS-7 cells showing cleavage products of ITE, which were stabilized by ALLN. Each lane was loaded with the same amount of cell extracts from parallel cultures treated or not treated with ALLN to ensure equal protein loading. Proteins were detected by anti-VSV antibodies. One of the stabilized cleavage products corresponded to the size of the entire intracellular domain (arrow) and another one to a smaller fragment (arrowhead).