Fig. 5. Identification of the CSP regions that associate with N-type calcium channels. CSP deletion mutants were immobilized on glutathione-agarose beads and incubated with recombinant _1B synprint His₆ at 37°C for 1 hour. The beads were washed, and bound proteins were eluted in sample buffer, fractionated by SDS-PAGE and subjected to western blot analysis. The monoclonal antibody used recognizes a sequence Thr-Leu-Tyr-Asp-Asp-Asp-Asp-Lys (Anti Express, Invitrogen) in the fusion proteins. These results are representative of 10 independent experiments.