Fig. 5. The regulatory effect of rBM on ER expression is not due to changes in growth rate. (A) Proliferation of Scp2 cells grown for 3 days on plastic or in the presence of rBM after adding different concentrations of insulin (in the absence of serum or other growth factor). Proliferation was determined as the percentage of BrdU-labeled cells by immunofluorescence. (B) ER protein levels in Scp2 cells grown in the presence of different insulin concentrations as indicated in A were evaluated by western blot (a, representative gel) and quantified by densitometry (b). ER expression did not correlate with the proliferation status of the cells, regardless of the substrata.n=4; *P<0.05 versus no insulin.