Fig. 2. Stably expressed Survivin-GFP, but not the epitope(s) recognized by the monoclonal antibody 8E2, can also be repressed by Survivin RNAi. (A,B) HeLa cells stably expressing Survivin-GFP (green) were subjected to Survivin RNAi and immunostained for tubulin (red) and counterstained with DAPI (DNA; blue). (A) Control cell with Survivin-GFP concentrated at the centromeres (arrows). (B) The Survivin-siRNA-transfected cell is depleted of Survivin-GFP signal. (C,C'-D,D') Control or Survivin-siRNA-transfected cells stained for Survivin using the polyclonal antibody (C,D in red) and centromeres (C',D' in red); the epitope(s) recognized by the monoclonal antibody 8E2 (green) and DNA (blue). Survivin-specific siRNA does not abolish spindle staining by 8E2. Bars, 5 µm.