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Fig. 2. Effect of calcium on trail formation by gliding parasites. Formation of gliding trails by parasites treated with (A) BAPTA-AM, (B) BAPTA, (C) a diluent control, (D) CaCl2, (E) EGTA in pH 6.0 low calcium Ringer's or (F) EGTA in pH 7.2 low calcium Ringer's. Coverslips were stained with a fluorescently conjugated antibody against the plasma membrane marker SAG1 to visualize trails. Images shown are representative of three independent experiments showing similar results. Bar, 10 µm.