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Fig. 5. Addition of H. pylori stimulates the expression of the 2.3 kb human MMP-7 promoter coupled to a luciferase reporter. (A) Stimulation of MMP-7-luc (1.0 µg well-1) by two different isolates of H. pylori, one vacA+ and cagA+ (E6), the other vacA- and cagA+ (E5). (B) Failure of H. pylori (60190) to stimulate MMP-7-luc when added to a filter over transfected AGS cells. (C) Stimulation of MMP-7-luc by the chemokine IL-8 (250 ng ml-1), proinflammatory cytokines TNF-{alpha} (2.5 ng ml-1) and IL-6 (5 ng ml-1), and the growth factor EGF (100 ng ml-1). (D) However, the effect of H. pylori (60190) on MMP-7-luc was not mediated by soluble AGS cell factors, because application of H. pylori to AGS cells on filters cultured above MMP-7-luc transfected AGS cells had no effect on MMP-7-luc compared with direct application to AGS cells. Means±s.e.m., n=3.