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Fig. 6. H. pylori-increased p50, p65 and c-fos activity in AGS cells mediates MMP-7-luc expression. (A) In western blots of nuclear extracts, H. pylori increased NF-{kappa}B p50 and p65 compatible with nuclear translocation. (B) In nuclear extracts of H. pylori-treated cells there was increased p65 and p50 binding to the NF-{kappa}B consensus cis-element. (C) The inhibitor of I{kappa}B degradation, BAY11-7082 (10 µM), depressed MMP-7-luc responses to H. pylori, as did the MEK inhibitor PD98059 (20 µM). (D) In nuclear extracts, there was also activation of c-fos, as indicated by binding to oligonucleotides with relevant consensus sequences. (E) Cotransfection with c-fos (0.6 µg well-1) increased expression of MMP-7-luc. Means±s.e.m., n=3.