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Fig. 7. Induction of MMP-7 by H. pylori is mediated by RhoA. (A) Expression of the MMP-7-luc plasmid (0.5 µg well-1) was increased in a time-dependent manner (hours after transfection) by cotransfection with plasmids encoding constitutively active forms of Rho (L63RhoA, 0.25 µg well-1) and, to lesser extent, Rac (L61Rac, 0.25 µg well-1); constitutively active cdc42 (L61cdc42, 0.25 µg well-1) had only a slight effect. (B) H. pylori-stimulated MMP-7-luc expression was inhibited by cotransfection with dominant-negative (DN) RhoA (N19RhoA, 0.5 µg well-1) and DN-Rac (N17Rac, 0.5 µg well-1). (C,D) L63RhoA-stimulated MMP-7-luc expression was inhibited by BAY11-7082 (10 µM) and by cotransfection with DN-jun vector (0.5 µg well-1). (E,F) L63Rac-stimulated MMP-7-luc expression was inhibited by BAY11-7082, but not by cotransfection with a DN-jun vector. Means±s.e.m., n=3.