Fig. 1. Specificity of antiserum for emerin in lmna^-/- MEFs. MEFs were extracted with a 1% SDS buffer and subjected to SDS-PAGE with prestained molecular weight markers (Sigma), as previously described (Manilal et al., 1996). The western blot was developed with emerin anti-serum (1/1000), peroxidase-labelled pig anti-rabbit Ig (DAKOpatts) and Supersignal chemiluminescent substrate (Pierce). Captured images of prestained blue markers (Mr; lane 1; sizes shown in kDa) and exposed film (Em; lane 2) were aligned after capture. The emerin band at 34 kDa is indicated. There are no higher molecular weight bands of other LEM domain proteins (LAP2 or MAN1). Pre-immune rabbit serum gave no bands at all (not shown).