Fig. 3. Some mutant lamins form nuclear foci in MEFs (lmna^-/-) and emerin co-localizes with foci formed by `rod' mutants but not `tail' mutants. Transfection of MEFs (lmna^-/-) with lamin A N195K (A), E358K (B) or L85R (C) produced nuclear foci that were also positive for emerin (L85R produced foci only rarely; Table 1). Similar results were obtained with R386K and M371K (not shown). Two of the three `tail' mutants, R453W (D) and R527P (E), produced nuclear foci only rarely and these did not accumulate emerin (arrows). Bars, 10 µm.