Fig. 5. -HIS2AV foci persist into late stages of meiotic prophase in okr and spnB mutants. Following region 3 in the germarium, each cyst enters the vitellarium, which is divided into several developmental stages (numbered 2-14). The meiotic nucleus condenses into a tight mass, the karyosome, meiosis arrests and the SC dissolves from the nucleus by stage 6. The remaining cells become polyploid nurse cells. The oocyte (arrows) is marked by the ORB protein (green), DNA is stained blue and the -HIS2AV foci are red. (A) A merge of five confocal sections from an okr^WS vitellarium showing foci in a stage 3 oocyte and their absence at stage 5. (B) A merge of three confocal sections from a spnB^BU vitellarium. The -HIS2AV foci are present in a stage 4 cyst but absent in the stage 6 cyst (see inset). Bar, 10 µm.