Fig. 2. Characterisation of WT-ACE and GPI-ACE expression in CHO and SH-SY5Y cells. CHO and SH-SY5Y cells were transfected with either empty pIRESneo vector (mock), WT-ACE cDNA or GPI-ACE cDNA. Cell lysates were prepared as described in Materials and Methods. (A) Lysates were immunoblotted using the ACE antibody, RP183. (B) Lysates were immunoblotted using a monoclonal ß-actin antibody. (C) Lysates were assayed for ACE activity with BzGly-His-Leu as substrate. Results are the mean ± s.d. of three experiments.