Fig. 6. Inhibition of the regulated shedding of WT-ACE and GPI-ACE by a range of hydroxamate-based inhibitors. CHO cells transfected with either WT-ACE or GPI-ACE were incubated for 7 hours in the presence of 1 µM PMA and 10 µM concentrations of a range of structurally variant hydroxamate-based inhibitors [numbers as in Parkin et al. (Parkin et al., 2002)]. The medium was then harvested and concentrated and samples were assayed for ACE activity with BzGly-His-Leu as substrate. Results are the mean ± s.d. of three experiments.