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Fig. 1. Subcellular localization of DYRK1A. (A) Schematic representation of a series of HA-tagged deletion mutants covering the full-length open reading frame of human DYRK1A (754 amino acids alternative spliced form). Representative domains are indicated: NLS (nuclear localization signal); KINASE (kinase catalytic domain); PEST (domain rich in proline, glutamate, serine and threonine); His (histidine repeat) and Ser/Thr (serine/threonine-rich region). {Delta}, deletion; N, nuclear accumulation; SP, speckle-like staining pattern. All these constructs were transiently transfected in COS-7 cells. (B) Western blot of total protein extracts (10% SDS-PAGE) using an anti-HA antibody for protein detection. Positions of marker proteins (in kDa) are indicated. (C) Indirect immunofluorescence analysis using an anti-HA monoclonal antibody, followed by an FITC-conjugated rabbit anti-mouse secondary antibody. Results for the most representative mutants are shown in B and C.