Fig. 2. Identification of a novel NLS in the catalytic domain of DYRK1A. The GFP-DYRK1A fusions indicated were expressed by transient transfection in COS-7 cells, and their subcellular localization was analyzed by fluorescence microscopy. The sequence and position of the second NLS motif predicted and the polybasic sequence element adjacent to it are indicated. The DYRK1A amino-acid residues of the different GFP fusions are also indicated.