Fig. 6. DYRK1A localization responds to the transcriptional state of the cell. COS-7 cells were transfected with GFP-fusion proteins of DYRK1A wildtype (A), the catalytically inactive mutant (B) and the histidine domain (C) and treated with -amanitin (50 µg/ml, 5 hours) to inhibit transcription. Speckles labeled with anti-SC35 became larger, rounded-up and overlapped with DYRK1A.