Fig. 5. Formation of clusterin aggresome induced relocalization of mitochondria. (A) COS-7 cells transfected with GFP--clu were incubated for 15 minutes with 100 nM MitoTracker Red^TM, fixed, counterstained with DAPI and then analysed by fluorescence microscopy. In cells presenting clusterin aggresomes, mitochondria collapsed and massively relocalized at the juxtanuclear aggresome. The same results were obtained with immunostaining using a COX6C antibody coupled with rhodamine-conjugated secondary antibody. Bar, 10 µm for MitoTracker Red and COX6C labeling. (B) The co-localization of mitochondria and GFP--clu is visible only at the level of large GFP--clu aggregates. Bar, 10 µm.