Fig. 2. Identification of MLCK and ROCK in human T cells. Western blot analysis of T cells probed with mAbs specific for MLCK (lane 2) and ROCK I (lane 3). Mouse lung lysate was used as a positive control for the smooth muscle and non-smooth muscle forms of MLCK (lane 1). Molecular weight markers of 220 kDa (myosin) and 97kDa (phosphorylase A) are indicated.