Fig. 3. LFA-1-mediated migration of T cells on ICAM-1. Time lapse video microscopy of human T cells treated with Mg²⁺/EGTA showing a sequence of initial contact with and migration on ICAM-1. Cells and medium were preheated to 37°C prior to recording. See Movie 1 for further examples of attachment, polarization and migration. The white arrows at time 0 and 45 seconds highlight the cells that have made initial contact with ICAM-1. Bar, 20 µm.