Fig. 4. Effect of inhibiting MLCK activity on T cell attachment and migration on ICAM-1. Phase images of T cells on (A) ICAM-1, (B) ICAM-1 in the presence of Mg²⁺/EGTA, (C) preincubated with 20 µM ML-7 and plated onto ICAM-1 with Mg²⁺/EGTA. Bar, 10 µm. Motility and migration as determined by time lapse microscopy (scale in µm). (D) T cells on ICAM-1 in the presence of Mg²⁺/EGTA (average speed=10.3 µm/minute; scale in µm). (E) T cells plated onto ICAM-1 in Mg²⁺/EGTA and allowed to migrate for 20 minutes followed by the addition of 20 µM ML-7 for 70 minutes (average speed=0.71 µm/minute). Green and red dots represent the starting and stopping positions of each individual T cell.