Fig. 6. Effect of inhibiting CaM activity on T cell attachment and migration on ICAM-1. (A) Proportion of T cells in presence of Mg²⁺/EGTA bound to ICAM-1 over an increasing dose range of CaM inhibitor, W-7 (n=4). (B) Motility and migration as determined by time lapse microscopy (scale in µm). As in Fig. 4, Mg²⁺/EGTA-stimulated T cells were plated onto ICAM-1 minus or plus pre-incubation with 50 µM W-7 and allowed to migrate for 30 minutes (average speed of 7.85±0.15 versus 1.53±0.05 µm/minute). Green and red dots represent the starting and stopping positions of each individual T cell. (C) Phase images of Mg²⁺/EGTA-stimulated T cells plated onto ICAM-1 and allowed to migrate for 30 minutes followed by the addition of 50 µM W-7 for 10 minutes. Bar, 20 µm.