Fig. 7. Effect of inhibiting ROCK activity on T cell attachment and migration on ICAM-1. Phase images of Mg²⁺/EGTA-stimulated T cells after 20 minutes of migration. (A) On ICAM-1 or pre-incubated with 10 µM Y-27632. (B) T cells, pre-incubated with C3 exoenzyme overnight at 37°C prior to plating on ICAM-1. (C) T cells were allowed to adhere to ICAM-1 in presence of Mg²⁺/EGTA for 20 minutes followed by the addition of 10 µM Y-27632. The morphological effects were recorded by time lapse microscopy over 15 minutes (bar, 20 µm). (D) T cells on ICAM-1 in the presence of Mg²⁺/EGTA (average speed=12.6±0.22 µm/minute). T cells were allowed to adhere for 20 minutes followed by the addition of 10 µM Y-27632 for 70 minutes (average speed=4.81±0.13 µm/minute; scale in µm). Green and red dots represent the starting and stopping positions of each individual T cell.