Fig. 8. Effect of MLCK, CaM and ROCK inhibitors on cell spreading and morphology. (A) Mg²⁺/EGTA-stimulated T cells were allowed to migrate on ICAM-1 or BSA for 20 minutes in the presence of either ML-7 (25 µM), W-7 (50 µM) or Y-27632 (20 µM) prior to fixation, permeabilization and staining for F-actin (n=6). Cell area was recorded by a LSC (6000 cells per cover slip). (B) T cell length was measured after incubation with either ML-7 (25 µM), W-7 (50 µM), Y-27632 (20 µM) or C3 exoenzyme (20 µg/ml) (n=18-34). All measurements were taken after the T cells were allowed to migrate for 20 minutes on ICAM-1 in the presence of Mg²⁺/EGTA. The dotted line represents the average diameter of T cells in suspension (included as a reference point). All results are expressed as an mean±s.d. P<0.01(^***).