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Fig. 1. Expression of Msi-1 mRNA in X. laevis intestine and stomach analyzed by RT-PCR. (A) Developmental changes during normal metamorphosis. Msi-1 mRNA is expressed transiently around stages 61-62. (B) Expression of Msi-1 mRNA in stage 57 tadpoles treated with 5 nM TH in vivo. Msi-1 mRNA becomes detectable around day 5. The two products of Msi-1 mRNA with different sizes are produced by alternative splicing (Kaneko et al., 2000). The products of EF-1{alpha} mRNA serve as a control.