Fig. 11. Dose-response of NMDA on DEA/NO-stimulated cGMP increases in 7 DIV (A) and 14 DIV (B) granule cells. Cells were incubated with the indicated concentrations of NMDA for 48 hours and then stimulated with vehicle (basal response, ) or 1 µM DEA/NO (), and their cGMP content determined as described previously. Results are expressed as mean ± s.e.m. of three experiments performed in triplicate. ^**P<0.01; ^***P<0.001. Corrections for possible cell loss were made by determining the total amount of protein in each well (10⁶ cells=0.1 mg of protein).