Fig. 3. Concentration- (A) and time- (B) dependence curves for DEA/NO-stimulated cGMP accumulation in cerebellar granule cells. 7 DIV cells were washed twice with Locke's solution and incubated in this medium for 1 hour. 0.5 mM IBMX was added to the incubation medium during the last 30 minutes of incubation. The cells were then stimulated with the indicated concentrations of DEA/NO for 10 minutes (A) or with 1 µM DEA/NO for the times indicated (B). Results are expressed as pmol/10⁶ cells corresponding to the mean ± s.e.m. of four experiments performed in triplicate using different cultures. Significant differences from control values (non-DEA/NO stimulated) are indicated: ^**P<0.01; ^***P<0.001.