Fig. 6. Expression pattern of the different NO_GCR subunits during cerebellum development (A) and relative contributions of ₁ and ₂ subunits to total mRNA in cerebellum at three developmental stages P7, P14 and P21. Equal amounts of mRNA were used for RT reactions and then real time PCR reactions were performed with specific primers as described before. These results correspond to the mean ± s.e.m. of four different experiments performed in triplicate.