Fig. 7. NMDA treatment regulates the expression of ₁, ₂ and ß₁ NO_GCR subunits mRNAs. 7 DIV or 14 DIV granule cells were incubated with vehicle (open bars), 100 µM NMDA (striped bars) for 24 hours. When the effect of 50 µM AP5 or 5 µM MK-801 on NMDA action was evaluated they were added to culture media 10 minutes before NMDA addition. After treatment, total RNA was isolated and used for quantitative RT-PCR. Equal amounts of mRNA were used to perform the RT reactions followed by quantitative PCR with the specific primers and probes for ₁, ₂, ß₁ and 18S rRNA. A, B and C represent the relative abundance of ₁, ß₁ and ₂ mRNA (white columns). These results were normalised using the values obtained for 18S rRNA and are the mean±s.e.m. of four experiments performed in triplicate. All the values refer to the amount of mRNA in 7 DIV cells. ^***P<0.001; ^**P<0.01; ^*P<0.05 are significantly different from the amount of mRNA corresponding to 7 DIV control cells; ^&&P<0.01 are significantly different from the amount of mRNA corresponding to 14 DIV control cells; ⁺⁺⁺P<0.001; ⁺⁺P<0.01; ⁺P<0.05 are significantly different from the amount of mRNA corresponding to NMDA-7 DIV-treated cells; ^$$P<0.01; ^$P<0.05 are significantly different from the amount of mRNA corresponding to NMDA-14 DIV-treated cells.