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Fig. 2. Short-chain ceramides decrease lipid order as measured by fluorescence anisotropy of DPH-PC in plasma membrane vesicles from RBL cells. Ceramides were added to DPH-PC-labeled vesicles (~150 µM phospholipids), and fluorescence anisotropy was measured subsequent to each addition. {blacksquare}, C2-dhcer; {bullet}, C2-cer; {blacktriangleup}, C6-cer. Results shown are average values of at least three experiments for each ceramide; bars designating s.e.m. are shown where larger than the width of data points.