Fig. 3. Western blot analysis of melanogenic protein glycosylation in subcellular fractions of wild-type and of uw-mutant melanocytes. Equal amounts of protein (0.1 µg/lane) from cell extracts, melanosomes and secreted vesicles of wild-type or uw-mutant melanocytes were electrophoretically separated by 8% SDS-PAGE. Tyrosinase or Tyrp1 were then detected immunochemically with PEP7 or PEP1 antibodies, respectively. Glycosylation of melanosomal proteins was determined following digestion with buffer (–), EndoH (E) or PNGaseF (P), as indicated.