Fig. 1. Effects of IGF-1 and EGF on keratinocyte spreading and rounding. (A) FACS analysis of expression of IGF-1 receptor (grey line) and EGFR (black line) in primary human keratinocytes after suspension culture. (B) Stimulation of keratinocyte spreading by IGF-1. Histograms show size distribution of 200 keratinocytes per sample plated in the presence of 100 ng ml^-1 IGF-1 (top), 10 ng ml^-1 EGF (middle) or 4 µM cytochalasin D and 100 ng ml^-1 IGF-1 (bottom). Grey bars indicate stimulated cells, black bars unstimulated cells. Left panels show microscopic pictures of stimulated keratinocytes at equal magnifications. (C) Stimulation of keratinocyte rounding by EGF. Histograms show size distribution of 200 keratinocytes per sample stimulated with 10 ng ml^-1 EGF (top, grey bars) or 100 ng ml^-1 IGF-1 (bottom, grey bars). Black bars in both histograms indicate unstimulated control cells.