Fig. 3. Expression of a constitutively active PI-3K mutant (rCD2p110) in primary human keratinocytes. (A) Western blot analysis with the anti rat CD2 antibody Ox34 of keratinocytes infected with retroviruses encoding rCD2p110 or control virus (neo). Arrow points to rCD2p110 protein. Equal loading of the lanes was checked by Coomassie blue staining. (B,C) Immunofluorescent staining with Ox34 (green) and TRITC-phalloidin (red). Green staining shows localization of rCD2p110 at protruding areas of the cell membrane.