Fig. 6. Effects of MEK-1 activity, IGF-1 and PI-3K activity on keratinocyte motility. (A,B) Trajectories of cells expressing puro (A) or MAPKK1 (B) followed for 24 hours, showing 20 cells per sample. Origin and end point for each cell are indicated by coloured dots (green/red). (C,D) Histograms showing speed of cell movement. Each bar represents a separate experiment, with 20 cells being analysed per experiment. The number of cells moving at the same speed within an experiment is represented by the width of the bar. Red lines connect the means of individual experiments. Mean and standard error of the mean for all replicate experiments are given above the bars. (C) Comparison between cells expressing puro and MAPKK1. (D) Comparison between puro-expressing or uninfected keratinocytes treated with 0.1% DMSO and MANA-expressing or uninfected keratinocytes treated with 25 µM PD98059 in the presence of 10 ng ml^-1 EGF. (E) Migration speed (µm h^-1) of keratinocytes left untreated or treated with 0.1% DMSO or 25 µM LY294002 and left unstimulated or stimulated with 100 ng ml^-1 IGF-1. (F) Keratinocytes were preincubated with 0.1% DMSO and 25 µM LY294002, and left unstimulated or were stimulated with 100 ng ml^-1 IGF-1. A similar result was obtained when 50 µM LY294002 were used. Bars in E,F show mean±s.e.m.