Fig. 7. Effect of growth factor signalling on wound epithelialization. Mitomycin-C-treated keratinocytes in FAD low Ca²⁺ were wounded and incubated for 6 hours in the presence or absence of growth factors. (A-F) Normal human epidermal keratinocytes were left untreated (A) or were treated with: (B) 10 ng ml^-1 EGF+ 100 ng ml^-1 IGF-1; (C) 100 ng ml^-1 IGF-1; (D) 100 ng ml^-1 IGF-1 µM and 25 µM LY294002; (E) 10 ng ml^-1 EGF; (F) 10 ng ml^-1 EGF and 10 µM PD98059. (G-J) primary human keratinocytes infected with retroviral vectors encoding: (G) empty vector puro; (H) MAPKK1; (I) empty vector neo; (J) rCD2p110.