Fig. 10. NDP kinase pellets with microtubules and endosomal vesicles. Prior to homogenization, cells were incubated in the absence and presence of 10 ng ml^–1 EGF for 15 minutes. Taxol-stabilized microtubules and associated membranes were then isolated as described in Materials and Methods, in the absence of nucleotides. Fractions were analysed by immunoblotting with antibodies to NDP kinase, tubulin and the endosomal markers Rab4 and LAMP-1.