Fig. 11. Nucleotides release NDP kinase from taxol-stabilized microtubules and associated vesicles. (A) An extract from resting cells was divided into equal portions that were incubated with Taxol in buffer supplemented or not with ATP or GTP (both at 1 mM); after washes, pellets were tested for NDP kinase, tubulin, Rab4 and LAMP-1. (B) As above; binding of NDP kinase to the MT/Ves pellets was assessed in samples where GTP or guanine nucleotide analogs were included in the incubation with taxol.