Fig. 3. Stimulation of cells with bombesin, a GPCR agonist, also induces translocation of NDP kinase to the actin-rich cell cortex. Serum-starved cells were treated with 10 nM bombesin for 10 minutes, then stained with Ab-1 antibody (a) and rhodamine-phalloidin (b). Arrows show accumulation of NDP kinase around vesicles. The arrowheads indicate patches where NDP kinase staining extends beyond the actin-rich ruffling edge. Scale bar, 25 µm.