Fig. 6. Limited overlap of Tiam1 and NDP kinase in ruffles. Serum-starved cells were treated with 10 ng ml^–1 EGF for 2 minutes. Cells were stained with anti-NDP kinase A (a) and anti-Tiam1 (b) antibodies; (c) phase contrast image. Notice that NDP kinase is present throughout the ruffle, whereas Tiam1 is confined to a small area (arrow). The merged image (d) highlights the limited overlap between the signals for Tiam1 and for NDP kinase. (c) Scale bar, 25 µm.