Fig. 8. Characterization of the vesicles labeled by NDP kinase. (A). Size distribution of NDP kinase-labeled vesicles. The histogram was built as described in Materials and Methods. (B) NDP kinase is associated with vesicles labeled by LAMP-1. This image shows the immunolocalization of NDP kinase with Ab-1 (red) and LAMP-1 (green) in quiescent cells. NDP kinase labels the periphery (a) of vesicles, whereas LAMP-1 is visible in the lumen (b, arrows); a circular ruffle (arrowhead) is stained only by NDP kinase. Scale bar, 25 µm.A large vacuole positive for both NDP kinase (c) and LAMP-1 (d) has a multivesicular appearance. Scale bar, 5 µm.