Fig. 9. Microtubular association of NDP kinase-labeled vesicles. Cells were fixed and double stained with antibodies to NDP kinase (Ab-1) and -tubulin after being serum starved for 18 hours (–serum), kept in complete medium (+serum) or treated with 33 µM nocodazole for 1 hour (nocodazole). Arrowheads show vesicular structures visible in phase contrast, which are labeled by antibodies to -tubulin and NDP kinase in serum-starved and serum-treated cells (left and middle, respectively). Labeling of vesicles is lost in cells treated with nocodazole (right). Scale bar, 20 µm.