Fig. 3. The effect of EP₃ receptor stimulation on the F-actin cytoskeleton in IMCD cells. IMCD cells were incubated with the indicated agents as described in the legend to Fig. 1. After completion of the incubations, cells were fixed, permeabilized and incubated with TRITC-conjugated phalloidin (0.1 mg/ml). Phalloidin fluorescence was detected by laser scanning microscopy. Shown are single representative cells from at least three independent experiments for each condition.