Fig. 3. Lack of PKP1 increases migration rates in response to in vitro wounding. Keratinocyte cell lines were seeded at high density and grown for 48 hours in 35 mm dishes prior to wounding. Time-lapse microscopy monitored migration over 24 hours and image analysis determined movement (µm/hour) between the given time points. Graph represents mean±s.e.m. (*P<0.05) using patient 1 (3-month-old male) and control 1 (31-year-old female) cells. Lower phase images taken after 16 hours, Black line indicates wound edge at time 0. Scale bar: 100 µm.